VCF demo

aGPCR demo

load demo

Via: “upload vcf”

Input:

rtel1.vcf

load demo

Via: “upload structure and mutations”

Input:

1) Upload PDB: 4DLQ, remove HETATMs
2) Initial mutation: A:804S
2b) Additional mutations of mut_0 (in explorer):
   A:815S, A:806A, A:803Y

Sequence alignment

load 1lib

Inputs:
1) Upload PDB ID: 1lib
2) Sequence alignment:

alignment.clw

Target sequence

load 1lib

Inputs:
1) Upload PDB ID: 1lib
2) Target sequence:

1lib_seq.fa